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KMID : 0362919930110010035
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Journal of the Korean Society for Therapeutic Radiology and Oncology
1993 Volume.11 No. 1 p.35 ~ p.42
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Frequency of Micronuclei in Lymphocytes Following Gamma and Fast-neutron Irradiations
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Kim Sung-Ho
Cho Chul-Koo
Yoo Seong-Yul
Koh Kyoung-Hwan
Kim Tae-Hwan
Chung In-Yong
Yun Hyong-Geun
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Abstract
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The dose response of the number of micronuclei in cytokinesis-blocked (CB) lymphocytes after in vitro irradiation with -rays and neutrons in the 5 dose ranges was studied for a heterogeneous population of 4 donors. One thousand binucleated cells were systematically scored for micronuclei. Measurements performed after irradiation showed a dose-dependent increase in micronuclei(MN) frequency in each of the doctors studied. The dose-response curves were analyzed by a linear-quadratic model, frequencies per 1000 CB cells were (0.31±0.049) D+(0.0022±0.0002) D2+(13.19±1.854) (r2=1.000, X2=0.7074, p=0.95) following irradiation, and (0.99±0.528) D+(0.0093±0.0047) D2+(13.31±7.309) (r2=0.996, X2=7.6834, p=0.11) following neutrons irradiation (D is irradiation dose in cGy). The relative biological effectiveness (RBE) of neutrons compared with -rays was estimated by best fitting linear-quadratic model. In the micronuclei frequency between 0.05 and 0.8 per cell, the RBE of neutrons was 2.37±0.17. Since the MN assay is simple and rapid, it may be a good tool for evaluating the y-ray and neutron response.
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KEYWORD
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Micronuclei, Gamma and neutron, Biological dosimetry, Cytokinesis blocked cell, Relative biological effectiveness
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